Influence of modeled microgravity on tobacco mosaic virus

  • N. P. Sus Institute of Agroecology and Environmental Management of NAAS
  • A. V. Orlovskyi Institute of Agroecology and Environmental Management of NAAS
  • O. A. Boyko National University of Life and Environmental Sciences of Ukraine
  • V. O. Tsvigun Institute of Agroecology and Environmental Management of NAAS
  • A. L. Boyko Institute of Agroecology and Environmental Management of NAAS
Keywords: TMV (tobacco mosaic virus), clinorotation, microgravity, inclusion bodies, viroplasm, gravisensitivity, cortical MT-associated ER sites

Abstract

Сlinorotation is an effective method of treating diseases caused by some plant viruses. Therefore, we researched the influence of microgravity (modeled by сlinorotation) on a tobacco mosaic virus (TMV) that infects many agricultural crops. It is known that cells of plants (infected with TMV) contain viral inclusion bodies or viroplasms and amount of viral inclusion bodies correlates with harmfulness of TMV. Therefore, the purpose of this study was to find out the effect of influence of modeled microgravity on inclusion bodies of TMV. In this study, we cultivated Nicotiana tabacum L. and inoculated them with TMV that was isolated from Norway maple (Acer platanoides L.). Then we divided these plants into two groups and cultivated these plants under normal and microgravitation conditions. Microgravity conditions were modeled by сlinorotation at 2 rpm for 4 hours a day. This experiment lasted 36 days. Changes of the amount of TMV inclusion bodies in cells of plants that cultivated under normal and microgravitation conditions was investigated by luminescence microscopy. We found that formation of TMV inclusion bodies under microgravitation conditions is first slowed down compared to formation under normal conditions and then their amount quickly decreasing. These results demonstrate the gravisensitivity of TMV. It was suggested hypothesis that this viroplasm pattern caused by the disorganization of cortical microtubule-associated ER sites (C-MERS) that are nodes of cellular transport pathways and nucleation centers of cortical microtubules and cortical microfilaments. It is known that under microgravity conditions there is a disorganization and disorientation of cortical microtubules, which stabilize C-MERS on which TMV viroplasms are formed. Thus, the disorganization and disorientation of cortical microtubules probably causes the disorganization of C-MERS, which leads to a decrease in the number of TMV viroplasms under the influence of microgravity. In this context, it is worth noting that some plant viruses, such as a Wheat streak mosaic virus (WSMV), a Potato virus M (PVM) and Potato curly dwarf virus (PCDV), are gravisensitive. These viruses belong to different taxa, for example WSMV belong to genus Tritimovirus (family Potyviridae), PVM belong to genus Carlavirus (family Betaflexiviridae) and PCDV belong to plant rhabdoviruses (uncertain taxonomic position), and differ both structurally and functionally. Therefore, the gravisensitivity of these viruses can occur by other mechanisms. Thus, antiviral therapeutic effect of clinorotation based on gravisensitivity of TMV and can be used in the production of virus-free seeds. To confirm this hypothesis, it is necessary to conduct a systematic review, as well as experimentally establish the fact of the disorganization of the C-MERS under microgravity conditions.

References

Boyko, A. L. (2003). Osnovy ekolohii ta biofizyky virusiv [Fundamentals of ecology and biophysics of viruses]. Fitosotsiotsentr, Kyiv (in Ukrainian).
Boiko, O. A., Taranenko, P. K., Bukhalo, A. S. (1992). Virusnaya bolezn shampinona pri intensivnoy tekhnologii ego vyrashchivaniya [Viral disease of agaric under intensive cultivation technology]. Dokl. AN Ukrainy, 8, 159–160 (in Russian).
Boyko, O., Shevchenko, T., Lukashov, D., Orlovska, G. (2014). The Circulation of Phytovirus among Plants and Fungy Involving the Helix Pomatia L. Problemy. Bulletin of Kyiv National Taras Shevchenko University, 17, 69–71.
Bulavin, I. V. (2015). Hravichutlyvist koreniv, utvorenykh z lystkovykh eksplantiv Arabidopsis thaliana v kulturi in vitro [Gravisensitivity of roots of Arabidopsis thaliana formed in vitro from leaf explants]. The Bulletin of Kharkiv National Agrarian University. Series Biology, 1, 6–13 (in Ukrainian).
Danilova, O. I. (2014). Doslidzhennia nespetsyfichnoi stiikosti roslyn pomidora do M- ta Y-virusiv kartopli [Investigation of nonspecific resistance of tomato plants to Potato virus M and Potato virus Y]. Naukovi dopovidi Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy, 6, 1–11 (in Ukrainian).
Didenko, L. F., Parkhomenko, N. I., Maksimenko, L. A., Dyachenko, N. S., Zaritskiy, N. M., Kozar, F. E. (1999). Vlyianye klynostatyrovanyia na vyrus kurchavoi karlykovosty kartofelia in vitro y in vivo [Influence of clinostating on the curly potato dwarf virus in vitro and in vivo]. Kosm. nauka tehnol., 5, 118–122 (in Russian).
Griffing, L. R., Lin, C., Perico, C., White, R. R., Sparkes, I. (2016). Plant ER geometry and dynamics: biophysical and cytoskeletal control during growth and biotic response. Protoplasma, 254, 43–56.
Heinlein, M. (2016). Viral Transport and Interaction with the Host Cytoskeleton. In: Kleinow T, ed. Plant–virus interactions: molecular biology, intra- and intercellular transport. Springer International Publishing, Switzerland, 39–67.
Kalinina, I. M. (2006). Mikrotrubochky v klitynakh epidermisu ta kory korenia Brassica rapa za umov klinostatuvannia [Microtubules in epidermal and cortical root cells of Brassica rapa under clinorotation]. Tsitol Genet., 40, 21–27 (in Ukrainian).
Korduum, E. L. (2009). Microgravity conditions – experimental basis used for the investigation of a gravity role in the plants’ ontogenesis process. Ecology and Noospherology, 20, 20–23.
Liu, C., Nelson, R. S. (2013). The cell biology of Tobacco mosaic virus replication and movement. Frontiers in Plant Science, 4, 1–10.
Mishchenko, L. T., Boyko, A. L., Chernyuk, S. O. (1999). Vplyv klinostatuvannia na pokaznyky serolohichnoho analizu virusu smuhastoi mozaiky pshenytsi v roslynakh Triticum aestivum [The influence of microgravitation on characteristics of viruses of striped mosaic wheat in Triticum aestivum]. Biopolym. Cell., 15, 319–323 (in Ukrainian).
Orlovskyi, A., Moroz, V., Boiko, A. (2016). Skryninh ta biolohichni vlastyvosti izoliativ VTM (Tobamovirus) na roslynakh platana skhidnoho (Platanus orientalis L.) ta klena hostrolystoho (Acer platanoides L.) [Screening and biological properties of ТМV isolates (Tobamovirus) on plants of eastern plane (Platanus orientalis L.) and Norway maple (Acer platanoides L.)]. Agroecological journal, 4, 133–139 (in Ukrainian).
Peña, E. J., Heinlein, M., 2013. Cortical microtubule-associated ER sites: organization centers of cell polarity and communication. Current Opinion in Plant Biology, 16, 764–773.
Romanchuk, S. M., 2010. Ultrastructure of statocytes and cells of the distal elongation zone in Arabidopsis thaliana under clinorotation. Cytology and Genetics, 44, 3–8.
Shevchenko, G. V., Kordyum, Е. L., 2012. Ispolzovanie transgennyih rasteniy Arabidopsis thaliana-GFP-ABD2 v eksperimentah po izucheniyu tsitoskeleta v usloviyah modelirovannoy mikrogravitatsii [Application of transgenic Arabidopsis thaliana-GFP-ABD2 plants in experiments for the investigation of cytoskeleton in simulated microgravity]. Kosm. nauka tehnol., 18, 51–56 (in Russian).
Soga, K., Wakabayashi, K., Kamisaka, S., Hoson, T., 2006. Hypergravity induces reorientation of cortical microtubules and modifies growth anisotropy in azuki bean epicotyls. Planta, 224, 1485–1494.
Ueda, H., Yokota, E., Kutsuna, N., Shimada, T., Tamura, K., Shimmen, T., Hasezawac, S., Doljae, V. V., Hara-Nishimura, I. (2010). Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells. Proceedings of the National Academy of Sciences, 107, 6894–6899.
Van Gisbergen, P. A. C., Bezanilla, M., 2013. Plant formins: membrane anchors for actin polymerization. Trends in Cell Biology, 23, 227–233.
Published
2018-11-28
How to Cite
Sus, N. P., Orlovskyi, A. V., Boyko, O. A., Tsvigun, V. O., & Boyko, A. L. (2018). Influence of modeled microgravity on tobacco mosaic virus. Ecology and Noospherology, 29(2), 138–141. https://doi.org/10.15421/031822