Variability at winter wheat varieties first generation which obtained mutagen action

  • M. M. Nazarenko Dnipro State Agrarian and Economic University
  • T. Y. Lykholat Oles Honchar Dnipro National University
Keywords: winter wheat, gamma-rays, depression, mutagenesis


The purpose of our investigation was to identify the features of the depressive effects of the aftereffect of different doses of gamma-rays on winter wheat varieties of local breeding in terms of germination, survival, morphometry, yield. The parameters of germination and survival, the passage of the main phases of ontogenesis in winter wheat plants of French varieties (Flamenko and Ghayta) at the first generation were studied. The influence of mutagenic depression on parameters of yield structure (morphometry of mature plants) was established and the level of their variability was estimated too. In 2019–2020, experiments were conducted in the research fields of the research center of the Dnieper State Agrarian and Economic University. The experiments used seeds of Flamenko and Ghayta varieties, irradiated with gamma rays in doses of 100, 150, 200, 250, 300 Gy. Control was dry seeds. The variety Ghayta can be classified as resistant to gamma-rays, the variety Flamenko corresponded to high-sensitive. Parameter of germination and survival were directly correlated with increasing dose, with a dose of 200 Gy already semi-lethal, a dose of 250 Gy for variety Flamenko was sublethal, 300 Gy doze full-lethal, for the variety Ghayta sublethal was 300 Gy dose by which almost no plant material was obtained. Such parameters as plant height, weight of grains from the main spike and weight of thousand grains, weight of grains from the plant, partially number of grain from the main spike reliably reproduce the mutagenic depression. Depression of the plants is affected by the genotype of the variety more than the dose of mutagen; the plant height parameter clearly demonstrates mutagenic depression. According to the results of factor and discriminant analysis as indicators affected by genotype-mutagenic interaction should be used germination and survival, pollen sterility, plant height, grain weight per spike, weight of thousand grains weight of grains from the plant. Doses of 100–200 Gy are optimal for further use to obtain mutations; it is possible to use a dose of 250 Gy for the variety Ghayta.


Asif, J. (2020). Effect of different pre-treatments on seed germination of Prosopis juliflora and Dalbergia sissoo: a step towards mutation breeding. Journal of forest science, 66, 80–88.

Çelik, Ö., Ekşioğlu, A. & Akdaş, E. Y. (2018). Transcript profiling of salt tolerant tobacco mutants generated via mutation breeding. Gene Expression Patterns, 29, 59–64.

Hallajian, M. T. (2016). Mutation Breeding and Drought Stress Tolerance in Plants. Drought Stress Tolerance in Plants, 2, 359–383.

Hiroyasu, Y. (2018). Mutation breeding of ornamental plants using ion beams. Breeding Science, 68(1), 71–78.

Kolakar, S. S., Nadukeri, S., Jakkeral, S. A., Hanumanthappa, M. & Gangaprasad, S. (2018). Role of mutation breeding in improvement of medicinal and aromatic crops: Review. Journal of Pharmacognosy and Phytochemistry, SP3, 425–429.

Li, H. J., Timothy, D. M., Mc Intoshc, R.A. & Zhou, Y. (2019). Wheat breeding in northern China: achievements and technical advances. The Crop Journal, 7(6), 718–729.

Nazarenko, M. (2015). Negativnyie posledstviya mutagennogo vozdeystviya [Peculiarities of negative consequences of mutagen action]. Ecological Genetics, 13(4), 25–26 (in Russian).

Nazarenko, M., Beiko, V. & Bondarenko, M. (2019). Induced mutations of winter wheat caused by gamma-rays fixed on plant height and stem structure. Agriculture and Forestry, 65(3), 75–83.

Nazarenko, M., Lykholat, Y., Grigoryuk, I., Khromykh, N. (2018). Optimal doses and concentrations of mutagens for winter wheat breeding purposes. Part I. Grain productivity. Journal of Central European Agriculture, 19(1), 194–205.

Saif-u-Malook, S. A., Qaisarani, M. K.,  Shabaz, H., Ahmed, M., Nawaz, M. & Qurban, A. (2015). Mutation breeding approach to breed drought tolerant maize hybrids. International Journal of Biosciences, 6(2), 427–436.

Shah, F., Adnan, M. & Basir, A. (2018). Global Wheat Production. Intechopen, London.

Shu, Q. Y., Forster, B. P. & Nakagava, H. (2013). Plant mutation breeding and biotechnology. CABI publishing, Vienna.

Spencer-Lopes, M. M., Forster, B. P. & Jankuloski, L. (2018). Manual on mutation breeding. Third edition. Food and Agriculture Organization of the United Nations, Rome.

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How to Cite
Nazarenko, M., & Lykholat, T. (2020). Variability at winter wheat varieties first generation which obtained mutagen action. Ecology and Noospherology, 31(2), 77-81.