Influence of phytoncide activity of Thuja occidentalis L. on strains of microorganisms under the action of car load (Dnipro city)

  • T. V. Sklyar Oles Honchar Dnipro National University
  • T. I. Yusypiva Oles Honchar Dnipro National University
  • O. O. Slobodyanyuk Oles Honchar Dnipro National University
Keywords: Thuja occidentalis L., phytoncide activity, needles, vehicle emissions

Abstract

Landscaping of modern cities is impossible without increasing the area of green planting, which performs the function of turning the environment into more health-friendly one. Especially valuable are the species of ornamental plants that have high volatile activity. Because volatiles regulate the balance of pathogenic microflora in the air of cities, they significantly improve people's living conditions. According to the results of a previous study it was found that Thuja occidentalis L. and Th. plicata Donn ex D. Don f. “Zebrina” had the highest bactericidal activity during the winter among members of the family Cupressaceae. Given the frost, wind, gas and smoke resistance of Th. occidentalis, the aim of the study was to investigate the dynamics of phytoncide activity of the needles of this tree species during the year in a relatively clean zone and under the influence of vehicle emissions on plants in the urban ecosystem of Dnipro. The material was collected in all seasons of the year at two monitoring points in Dnipro, which differ in the level of environmental pollution by vehicle emissions. Traffic intensity was determined by the Potichuk & Pilipaka method. Antimicrobial action of Th. occidentalis was studied by the method of placing the crops of microbial test cultures under the effect of volatile secretions of needles and evaluating the result on a scale of phytoncide activity. The test subjects were Staphylococcus epidermidis, Staphylococcus aureus, Micrococcus sp., Bacillus subtilis, Esherechia coli, Pseudomonas fluorescens, Candida albicans. Studies have shown that in a relatively clean area and under the action of traffic loads, the inhibitory effect of needles on the studied strains of microorganisms changes during the year: in winter it is the smallest, in spring begins to grow, in summer is maximum, and in autumn decreases again. Under the man-made growth conditions, the greatest volatile activity of Th. оccidentalis performs on test objects such as: S. aureus, Micrococcus sp. and E. coli. When affected by car load, there is significant inhibition of phytoncide production by needles in the presence of C. albicans, to a lesser extent – Ps. fluorescens and B. subtilis. It is recommended to use Th. оccidentalis in landscaping of urban areas with moderate traffic.

References

Akimov, Yu. A., Ostapchuk, I. F. (1985). Dejstvie e`firny`kh masel na patogennuyu mikrofloru organov dy`khaniya [The effect of essential oils on the pathogenic microflora of the respiratory system]. IV Symposium on Oil Plants and Oils, Simferopol, 2, 42 (in Russian).


Bakulin, V. T., Chindyaeva, L. N., Tsybulya, N. V. (2010). Antymykrobnaia aktyvnost lystev topolei y yv (Salicaceae) v Sybyry [Antimicrobic activity of poplar and willow (Salicaceae) leaves in Siberia]. Problems of Regional Ecology, 6, 60–64 (in Russian).


Bessonova, V. P., Ponomaryova, O. A. (2017).  Morfometrychni pokaznyky ta vmist plastydnykh pihmentiv khvoi Picea pungens zalezhno vid vidstani do avtoshliakhu [Morphometric characteristics and the content of plastid pigments of the needles of Picea pungens depending on the distance from the highways]. Biosystems Diversity, 25(2), 96–101.


Chao, S. C., Young, D. G., Oberg, C. J. (2000). Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. J. Essent. Oil Res., 12, 639–649.


Dolzhenkova, O. V., Petrova, V. N. (2007). Vply`v avtomobil`nogo transportu na bezpeku zhy`ttyediyal`nosti meshkanciv m. Dnipropetrovs`ka [Impact of road transport on the safety of residents of Dnipropetrovsk]. Bulletin of the Kharkiv National Highway University, 36, 56–60 (in Russian).


Ekolohichnyi pasport mista Dnipro (2016 r.) [Ecological passport of the Dnipro Sity (2016)] (in Ukrainian).


http://www.ieenas.org/p/tuia-zakhidna/


Iusypiva, T., Miasoid, G. (2016). The Impact of SO2 and NO2 Industrial Emissions on Anatomical Stem of Salix alba. International Letters of Natural Sciences, 51, 6–13.


Janssen, A. M., Chin, N. L. J., Scheffer, J. J. C., Baerheim Svendsen A. (1986). Screening for antimicrobial ctivity of some essential oils by the agar overlay technique. Statistics and correlations // Pharmaceutisch Weekblad, 8, 6, 289–292.


Kima, H., Leeb, B., Yunc, K. W. (2013). Comparison of chemical composition and antimicrobial activity of essential oils from three Pinus species. Industrial Crops and Products, 44, 323–329.


Laireiter, C. M., Schnabel, T., Kȍck, A., Stalzer, P., Petutschnigg, A., Oostingh, G. J., Hell, M. (2013). Active anti-microbal effects of larch and pine wood on bacterial strains. BioResources, 9 (1), 273–281.


Pasichnyy, H. V., Serdyuk, V. M., (2002). Dynamika vazhkykh metaliv v gruntovomu pokryvi u zvyazku z tekhnohennym zabrudnennyam otochuyuchoho seredovyshcha (na prykladi m. Dnipropetrovs'ka) [Dynamics of Heavy Metals in the Soils is Dealt with the Technogenic Pollution of Environment (for Example of Dnepropetrovsk City)]. Eсology & Natural Resource Management, 4, 111–117 (in Ukrainian).


Potichuk, O. V., Pilipaka, O. V. (2013). Guidelines for the implementation of the calculation and graphic work on the topic "Traffic characteristics and parameters of traffic flows" in the discipline "Monitoring traffic flows". NUPGP, Rivne. (in Ukrainian).


 


Sari Metsamuuronen, Heli Siren. Bioactive phenolic compounds, metabolism and properties: a review on valuable chemical compounds in Scots pine and Norway spruce. Phytochem Rev, 18, 623–664.


Slepyih, V. V. (2004). Prirodny`e i antropogenny`e faktory` i fitonczidnaya aktivnost` drevesny`kh porod. [Natural and anthropogenic factors and phytoncide activity of woody plants]. Forestry gardening, 6, 17–19 (in Russian). 


Tokin, B. P. (1980). Czelebny`e yady` rastenij. Povest` o fitonczidakh. [Healing poisons of plants. The tale of phytoncides]. Izd-vo Leningr. universiteta, Leningrad (in Russian).


Tsybulya, N. V. (2001). Metodika opredeleniya fitonczidnoj aktivnosti intaktny`kh rastenij [Methods of phytoncideactivity estimation in intact plants]. Plant Resources, 2, 106–115 (in Russian). 


Tsytsyura, N. І., Holovatuk, L. M. (2016). Likuvalni vlastyvosti predstavnykiv rodyny Cupressaceaе F. Neger // Investigations of medical plants – theoretical and practical aspects : Proceedings of 2th International scientific and practical Internet-Conference. Kharkiv, 260–261 (in Ukrainian).


Vainio-Kailia, T., Kyyhkynen, A., Rautkari, L., Siitonen, A. (2015). Antibacterial effects of extracts of Pinus sylvestris and Picea abies against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Streptococcus pneumoniae. BioResources, 10 (4), 7763–7771.


Volodarets, S., Gluchov, A. & Zaitseva, I. (2018). Phytoncide activity of woody plants under the conditions of steppe zone. Ekologia (Bratislava), 37(3), 219–229.


Yusypiva, T. I. (2018). Stan sosnovykh nasadzhen rekreatsiinoi zony poblyzu Prydniprovskoi TES ta yikh rol v ozdorovlenni miskoho seredovyshcha [The state of pine green planting in the recreational area near the Prydniprovsk TPP and their role in transforming the urban environment into the health-friendly one]. Biological, medical and scientific-pedagogical aspects of human health: Proceedings of International scientific and practical Internet-Conference. Poltava, Ukraine, 239 (in Ukrainian).


Yusypiva, T. I., Slobodyanyuk, O. O. (2019). Bakterytsydna aktyvnist khvoinykh introdutsentiv v umovakh m. Dnipro [Bactericidial activity of coniferous introduced species in the conditions of Dnipro]. Plants and Urbanization: Proceedings of VII International scientific and practical conference. Dnipro, Ukraine, 110–111 (in Ukrainian).


Zaitseva, I. O., Opanasenko, V. F. (2008). Putivny`k po botanichnomu sadu DNU. [Guide to the Botanical Garden of DNU]. DNU Press, Dnipro. (in Ukrainian).


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Published
2020-11-23
How to Cite
Sklyar, T., Yusypiva, T., & Slobodyanyuk, O. (2020). Influence of phytoncide activity of Thuja occidentalis L. on strains of microorganisms under the action of car load (Dnipro city). Ecology and Noospherology, 31(2), 87-92. https://doi.org/https://doi.org/10.15421/032014