Evaluation of the influence of heavy metals on the degree of damage Cameraria ohridella Deschka & Dimič, 1986 leaf surface of Aesculus hippocastanum Linnaeus, 1753

  • O. V. Seliutina Oles Honchar Dnipro National University, Dnipro, Ukraine
  • K. K. Holoborodko Oles Honchar Dnipro National University, Dnipro, Ukraine
Keywords: impact of invasive species, Cameraria ohridella, Aesculus hippocastanum sustainability, urban park ecosystems, urban environment


The work presents the results of research on effect of heavy metals (Zn, Cu, Pb, Cd) on degree of horse chestnut (Aesculus hippocastanum Linnaeus, 1753) leaf surface damage by chestnut miner (Cameraria ohridella Deschka & Dimič, 1986) caterpillars. Analysis of leaf blade damage of the forage plant species by C. ohridella was conducted for four years (2017–2021). The research covered all the main park urban ecosystems in Dnipro city where horse chestnut trees were planted. Monitoring was carried out during the three summer months, which corresponds to the main feeding period of the three most active C. ohridella generations (I, II, and III). About 96 samples were used and prepared for atomic absorption analysis to study the content of heavy metals in A. hippocastanum leaf blades. On the territory of the park ecosystems in Dnipro city, eight groups of model horse chestnut trees of 20–30 years of age were identified which have similar morphological and taxonomic features but different degrees of leaf damage by C. ohridella miner. Since the plantations of horse chestnut are mainly located in an urban environment, we conducted research to test the hypothesis about the influence of heavy metals on C. ohridella vital activity. Presumably, the content of heavy metals also affects the degree of leaf blade damage. In this regard, we conducted research on the content of heavy metals in A. hippocastanum leaf tissues. It was found that accumulation in the horse chestnut leaf tissues of four basic heavy metals (Zn, Cu, Pb, Cd) contained in all urban environments depends on the orographic features of the area where A. hippocastanum trees grow. It also was found that the higher in relief were located the horse chestnut plantings, the greater the percentage of their invasion by the miner, and this trend was observed during the three summer months. For example, in the Manuylivsky Park where horse chestnut trees are located at 56 m above sea level, the average leaf invasion by the miner in August is 14%; in the same month in Oles Honchar Dnipro National University Botanical Garden (149 m), the level of invasion was 78%. Research on the effects of heavy metals on C. ohridella populations also showed significant differences by the types of urban ecosystems. It was found that the higher content of heavy metals in the leaf tissues in a forage plant, the greater percentage of their surface damage by mines.


Augustin, S., Guichard S., Svato, A., Gilbert M. (2004). Monitoring the regional spread of the invasive leafminer Cameraria ohridella (Lepidoptera: Gracillariidae) by damage assessment and pheromone trapping. Environmental Entomology. 33, 1584–1592.

Augustin, S., Guichard, S., Heitland, W., Freise, J., Svatoš, A., Gilbert, M. (2008). Monitoring and dispersal of the invading Gracillariidae Cameraria ohridella. Journal of Applied Entomology, 133(1), 58–66.

Behmer, S. T. (2009). Insect herbivore nutrient regulation. Annual Review Entomology, 54, 165–187.

Deschka, G., Dimič, N. (1986). Cameraria ohridella n. sp. Aus Mactdonien, Jugoslawien (Lepidoptera, Gracillaridae). Acta Entomologica Jugoslavica, 22(1), 11–23.

Holoborodko, K. K, Ryabka, K. O, Zaitseva, I. A, Kondratieva, K. V. (2009). Poshyrennya ta suchasnyy stan kashtanovoyi minuyuchoyi moli (Cameraria ohridella Deschka & Dimič, 1986) u m. Dnipropetrovsʹk [Distribution and current state of chestnut moth (Cameraria ohridella Deschka & Dimič, 1986) in Dnepropetrovsk]. Problems of bioindication and ecology 14 (2): 163–168 (in Ukrainian).

Holoborodko, K. K., Rusynov, V. I., Seliutina, O. V. (2018). Invaziyni moli-strokatky (Gracillariidae Stainton, 1854) fauny Botanichnoho sadu Dniprovsʹkoho natsionalʹnoho universytetu imeni Olesya Honchara [Invasive moths (Gracillariidae Stainton, 1854) of the fauna of the Botanical Garden of Oles Honchar Dnipro]. Issue of steppe forestry and forest reclamation of soils, 47, 87–91 (in Ukrainian).

Jagiełło, R., Baraniak, E., Karolewski, P., Łakomy, P., Behnke-Borowczyk, J., Walczak, U., Giertych M. J. (2017). Ecophysiological aspects of the interaction between Cameraria ohridella and Guignardia aesculi on Aesculus hippocastanum. Dendrobiology, 78, 146–156.

Jagiеllo, E., Baraniak, E., Guzicka, M., Karolewsk, P., Ukowski, A., Giertych M. J. (2019). One step closer to understanding the ecology of Cameraria ohridella (Lepidoptera: Gracillariidae): The effects of light conditions. European Journal of Entomology, 116, 42–51.

Percival, G. C., Barrow, I., Noviss, K., Keary, I., Pennington, P. (2011). The impact of horse chestnut leaf miner (Cameraria ohridella Deschka and Dimic) on vitality, growth and reproduction of Aesculus hippocastanum L. Urban For. Urban Green. 10. 11–17.

Pschorn-Walcher, H. (1994). Field biology of the introduced horse-chestnut leafminer Cameraria ohridella Deschka et Dimič (Lepidoptera: Gracillariidae) in theVienna Woods. Linzer Biol. Beitr. 26, 633–642.

Reinhardt, F, Herle, M, Bastiansen, F, Streit, B. (2003). Economic impact of the spread of alien species in Germany. Berlin, Germany: Federal Environmental Agency (Umweltbundesamt). 229 pp.

Seliutina, O. V., Shupranova, L. V., Holoborodko, K. K., Shulman, M. V., Bobylev, Y. P. (2020). Effect of Cameraria ohridella on accumulation of proteins, peroxidase activity and composition in Aesculus hippocastanum leaves. Regulatory Mechanisms in Biosystems. 11 (2), 299–304.

Shupranova, L. V., Holoborodko, K. K., Seliutina, O. V., Pakhomov, O. Y. (2019). The influence of Cameraria ohridella (Lepidoptera, Gracillariidae) on the activity of the enzymatic antioxidant system of protection of the assimilating organs of Aesculus hippocastanum in an urbogenic environment. Biosyst. Divers., 2019, 27 (3), 238–243.

Skuhravy, V. (1998). Zur Kenntnis der Blattminen-Motte Cameraria ohridella Deschka & Dimic (Lep. Gracillaridae) an Aesculus hyppocastanum L. in der Tchechischen republiik. Ans fur Schadlingskunde Pflanz. Umweltschutz., 71, 8284.

Straw, N., Bellet-Travers, M. (2004). Impact and management of the horsechestnut leaf-miner (Cameraria ohridella). Arboricultural Journal, 28, 67–83.

Szaboky, C., Vas, J. (1997). Ujabb adatok a Vadgesztenyelevel-Aknazo-Moryrol Cameraria ohridella Deschka & Dimic 1986, Lep., Lithocolletidae. Novenyvedeiem., 33 (1), 29–33.

Tarwacki, G., Bystrowski, C., Celner-Warole, K. (2012). Effect of sun-exposure of the horse chestnut (Aesculus hippocasta-num L.) on the occurrence and number of parasitoids of the horse chestnut leafminer (Cameraria ohridella Deschka et Dimic) in central Poland in 2004–2006. Folia Forestalia Polonica, 54 (1), 5662.

Zerova, M. D., Nikitenko, G. N., Narolskiy, N. B., Gershenzon, Z. S., Sviridov, S. V., Lukash, O. V., Babidorich, M. M., (2007). Kashtanovaya miniruyushchaya mol' v Ukraine [Chestnut mining moth in Ukraine], Kiev, 90 (in Russian).

War, A. R., Taggar, G. K., Hussain, B., Taggar, M. S., Nair, R. M. & Sharma, H. C. (2018). Plant defence against herbivory and insect adaptations. AoB PLANTS, 10(4).

Abstract views: 21
PDF Downloads: 18
How to Cite
Seliutina, O., & Holoborodko, K. (2021). Evaluation of the influence of heavy metals on the degree of damage Cameraria ohridella Deschka & Dimič, 1986 leaf surface of Aesculus hippocastanum Linnaeus, 1753. Ecology and Noospherology, 32(1), 22-27. https://doi.org/https://doi.org/10.15421/032104