Chemical constituents and antimicrobial ability of essential oil from the fruits of Lonicera maackii (Rupr.) Maxim.

N. O. Khromykh; Y. V. Lykholat; O. O. Didur; T. V. Sklyar; A. O. Anischenko; T. Y. Lykholat

doi:10.15421/032206

N. O. Khromykh Oles Honchar Dnipro National University
Y. V. Lykholat Oles Honchar Dnipro National University
O. O. Didur Oles Honchar Dnipro National University
T. V. Sklyar Oles Honchar Dnipro National University
A. O. Anischenko Oles Honchar Dnipro National University
T. Y. Lykholat Oles Honchar Dnipro National University

Keywords: honeysuckle, essential oil, hydro distillation, phytochemicals, secondary metabolites, bioactivity

Abstract

Essential oils are the natural products that can pave the way for the creation of effective drugs with various beneficial properties. Plants of Lonicera maackii are well known in the natural growth regions of temperate Asia due to therapeutic effects and a high content of the bioactive compounds. Species diversity of steppe vegetation is enriched by the introduction of plants, but the assessment of this process effectiveness should also take into account the realization of plant genetic potential for the accumulation of secondary metabolites in the new growth conditions. The aim of the work was to establish whether the plants of L. maackii introduced in the steppe zone could reproduce the health promoting properties. The essential oil from the fruits of L. maackii was obtained by hydro distillation method. Chloroform and ethyl acetate fractions isolated from the aqueous distillate of L. maackii fruits were analyzed by gas chromatography combined with mass spectrometry (GC-MS analysis). Chemical constituents identified in ethyl acetate and chloroform fraction represented 98.6% and 94.2 % of the total compounds content, respectively. Among the most abundant components were hexadecane, acetic acid, butyl ester, hexadecanoic acid, methyl ester, linoleic acid, and linoleic acid methyl ester in the and the alkanes heptacosane, hexadecane, and tetradecane in chloroform fraction. Linoleic acid and some minor constituents of L. maackii fruit essential oil, such as ethyl-isoallocholate, arachidic acid, linalool and d-mannitol-1-decylsulfonyl are well known because of their reported bioactivities. The antimicrobial activity of essential oil was assessed against several clinical and collection bacterial and fungal strains. The results of disc-diffusion assays showed low to moderate activity of L. maackii fruit essential oil against Gram-positive and Gram-negative bacterial strains, as well as against fungal itraconazole-resistant clinical strain of Candida albicans. Thus, the essential oil derived from Lonicera maackii fruits may serve as potential resource of bioactive compounds having beneficial properties, in particular, the ability to overcome the resistance of pathogenic microorganisms to drugs. Further research is needed to identify the full range of bioactivities of the essential oil obtained from honeysuckle fruits.

References

Abu-Darwish, M. S., Cabral, C., Gonçalves, M. J., Cavaleiro, C., Cruz, M. T., Zulfiqar, A., Khan, I. A., Efferth, T., & Salgueiro, L. (2016). Chemical composition and biological activities of Artemisia judaica essential oil from southern desert of Jordan. Journal of ethnopharmacology, 191, 161–168.

Banu, H. R., & Nagarajan N. (2013). GC-MS determination of bioactive components of Wedelia chinensis (Osbeck) Merrill. Journal of Chemical and Pharmaceutical Research, 5(4), 279–285.

Bhimba, B. V., Pushpam, A. C., Arumugam, P., & Prakash, S. (2012). Phthalate derivatives from the marine fungi Phoma herbarum VB7. International Journal of Biological & Pharmaceutical Research, 3(4), 507–512.

Campana, R., Tiboni, M., Maggi, F., Cappellacci, L., Cianfaglione, K., Morshedloo, M. R., Frangipani, E., & Casettari, L. (2022). Comparative analysis of the antimicrobial activity of essential oils and their formulated microemulsions against foodborne pathogens and spoilage bacteria. Antibiotics (Basel, Switzerland), 11(4), 447.

Chouhan, S., Sharma, K., & Guleria, S. (2017). Antimicrobial activity of some essential oils – present status and future perspectives. Medicines, 4, 58.

De-Montijo-Prieto, S., Razola-Díaz, M. d. C., Gómez-Caravaca, A. M., Guerra-Hernandez, E. J., Jiménez-Valera, M., Garcia-Villanova, B., Ruiz-Bravo, A., Verardo, V. (2021). Essential oils from fruit and vegetables, aromatic herbs, and spices: composition, antioxidant, and antimicrobial activities. Biology, 10, 1091.

Diab, T. A., Donia, T., & Saad-Allah, K. M. (2021). Characterization, antioxidant, and cytotoxic effects of some Egyptian wild plant extracts. Beni-Suef University Journal of Basic and Applied Sciences, 10, 13.

Dilika, F., Bremner, P. D., & Meyer, J. J. (2000). Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatum: a plant used during circumcision rites. Fitoterapia, 71(4), 450–452.

Diniz do Nascimento, L., Moraes, A., Costa, K., Pereira Galúcio, J. M., Taube, P. S., Costa, C., Neves Cruz, J., de Aguiar Andrade, E. H., & Faria, L. (2020). Bioactive natural compounds and antioxidant activity of essential oils from spice plants: new findings and potential applications. Biomolecules, 10(7), 988.

Dr. Duke's Phytochemical and Ethnobotanical Databases (2022). U.S. Department of Agriculture, Agricultural Research Service. 1992–2016. http://phytochem.nal.usda.gov/ Accessed 17 May 2022

Du, X. Q., Shi, L. P., Cao, W. F., Chen, Z. W., Zuo, B., & Hu, J. Y. (2021). Add-on effect of honeysuckle in the treatment of coronavirus disease 2019: A systematic review and meta-analysis. Frontiers in pharmacology, 12, 708636.

Easwaran, L., & Ramani, A. V. (2014). Phytochemical examination and GC–MS studies of the medicinal plant Naravelia zeylanica. International Journal of Research and Development in Pharmacy and Life Sciences, 3(5), 1180–1188.

Fang, Z., Li, J., Yang, R., Fang, L., & Zhang, Y. (2020). A review: the triterpenoid saponins and biological activities of Lonicera Linn. Molecules, 25(17), 3773.

Ge, L., Xie, Q., Jiang, Y., Xiao, L., Wan, H., Zhou, B., Wu, S., Tian, J., & Zeng, X. (2022). Genus Lonicera: new drug discovery from traditional usage to modern chemical and pharmacological research. Phytomedicine: International Journal of Phytotherapy and Phytopharmacology, 96, 153889.

Khan, M., Khan, S. T., Khan, N. A., Mahmood, A., Al-Kedhairy, A. A., & Alkhathlan, H. Z. (2018). The composition of the essential oil and aqueous distillate of Origanum vulgare L. growing in Saudi Arabia and evaluation of their antibacterial activity. Arabian Journal of Chemistry, 11(8), 1189–1200.

Khromykh, N., Lykholat, Y., Shupranova, L., Kabar, A., Didur, O., Lykholat, T., & Kulbachko, Y. (2018). Interspecific differences of antioxidant ability of introduced Chaenomeles species with respect to adaptation to the steppe zone conditions. Biosystems Diversity, 26(2), 132–138.

Kumar, N. N., Ramakrishnaiah, H., Krishna, V., Deepalakshmi, A. P. (2015). GC-MS analysis and antimicrobial activity of seed oil of Broussonetia papyrifera (L.) Vent. International Journal of Pharmaceutical Sciences and Research, 6(9), 3954–3960.

Lan, W., Lin, S., Li, X., Zhang, Q., & Qin, W. (2017). Chemical composition of the leaf and stem essential oil of Adenophorae radix. AIP Conference Proceedings, 1820, 030001.

Lee, J. H., Lee, Y. Y., Lee, J., Jang, Y. J., & Jang, H. W. (2021). Chemical composition, antioxidant, and anti-inflammatory activity of essential oil from Omija (Schisandra chinensis (Turcz.) Baill.) produced by supercritical fluid extraction using CO2. Foods (Basel, Switzerland), 10(7), 1619.

Li, M., Wang, Y., Jin, J., Dou, J., Guo, Q., Ke, X., Zhou, C., & Guo, M. (2021). Inhibitory activity of honeysuckle extracts against influenza a virus in vitro and in vivo. Virologica Sinica, 36(3), 490–500.

Lykholat, Y. V, Khromykh, N. O., Lykholat, T. Y., Didur, O. O., Lykholat, O. A., Legostaeva, T. V., Kabar, A. M., Sklyar, T. V., Savosko, V. M., Kovalenko, I. M., Davydov, V. R., Bielyk, Y. V., Volyanik, K. O., Onopa, A. V., Dudkina, K. A., & Grygoryuk, I. P. (2019). Industrial characteristics and consumer properties of Chaenomeles Lindl. fruits. Ukrainian Journal of Ecology, 9(3), 132–137.

Maffei, M. E., Gertsch, J., & Appendino, G. (2011). Plant volatiles: production, function and pharmacology. Natural Product Reports, 28, 1359–1380.

Maggio, A., Rosselli, S., & Bruno, M. (2016). Essential oils and pure volatile compounds as potential drugs in Alzheimer’s disease therapy: an updated review of the literature. Current Pharmaceutical Design, 22(26), 4011–4027.

Mitić, Z. S., Jovanović, B., Jovanović, S. C., Mihajilov-Krstev, T., Stojanović-Radić, Z. Z., Cvetković, V. J., Mitrović, T. L., Marin, P. D., Zlatković, B. K., & Stojanović, G. S. (2018). Comparative study of the essential oils of four Pinus species: Chemical composition, antimicrobial and insect larvicidal activity. Industrial Crops and Products, 111(1), 55–62.

Pateiro, M., Munekata, P. E. S., Sant'Ana, A. S., Domínguez, R., Rodríguez-Lázaro, D., & Lorenzo, J. M. (2021). Application of essential oils as antimicrobial agents against spoilage and pathogenic microorganisms in meat products. International Journal of Food Microbiology, 337, 108966.

Pereira, I., Severino, P., Santos, A. C., Silva, A. M., & Souto, E. B. (2018). Linalool bioactive properties and potential applicability in drug delivery systems. Colloids and Surfaces B: Biointerfaces, 171, 566–578.

Pinto, M., Araújo, S. G., Morais, M. I., Sá, N. P., Lima, C. M., Rosa, C. A., Siqueira, E. P., Johann, S., & Lima, L. (2017). Antifungal and antioxidant activity of fatty acid methyl esters from vegetable oils. Anais da Academia Brasileira de Ciências, 89(3), 1671–1681.

Seow, Y. X., Yeo, C. R., Chung, H. L., & Yuk, H. G. (2014). Plant essential oils as active antimicrobial agents. Critical reviews in food science and nutrition, 54(5), 625–644.

Sosa, A. A., Bagi, S. H., & Hameed, I. H. (2016). Analysis of bioactive chemical compounds of Euphorbia lathyrus using gas chromatography-mass spectrometry and Fourier-transform infrared spectroscopy. Journal of Pharmacognosy and Phytotherapy, 8(5), 109–126.

Vergis, J., Gokulakrishnan, P., Agarwal, R. K., & Kumar, A. (2015). Essential oils as natural food antimicrobial agents: a review. Critical reviews in food science and nutrition, 55(10), 1320–1323.

Walters, D., Raynor, L., Mitchell, A., Walker, R., & Walker, K. (2004). Antifungal activities of four fatty acids against plant pathogenic fungi. Mycopathologia, 157(1), 87–90.

Xianfei, X., Xiaoqiang, C., Shunying, Z., & Guolin, Z. (2007). Chemical composition and antimicrobial activity of essential oils of Chaenomeles speciosa from China. Food Chemistry, 100(4), 1312–1315.

Yang, Y., Olatunde, O. Z., Yong, J., & Lu, C. (2018). Progress of chemical components and biological activities of Lonicera Maackii. Mathews Journal of Cancer Science, 3(1), 016.

Yong, J., Lu, C., & Huang, S. (2014). Chemical constituents of Lonicera maackii. Chemistry of Natural Compounds, 50(5), 945–947.

Zeng, W. C., Zhu, R. X., Jia, L. R., Gao, H., Zheng, Y., & Sun, Q. (2011). Chemical composition, antimicrobial and antioxidant activities of essential oil from Gnaphlium affine. Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association, 49(6), 1322–1328.

Zhou, H. Y., Zhao, N. N., Du, S. S., Yang, K., Wang, C. F., Liu, Z. L, & Qiao, Y. J. (2012). Insecticidal activity of the essential oil of Lonicera japonica flower buds and its main constituent compounds against two grain storage insects. Journal of Medicinal Plants Research, 6(5), 912–917.

Zuzarte, M., Correia, P., Alves-Silva, J. M., Gonçalves, M. J., Cavaleiro, C., Cruz, T., & Salgueiro, L. (2021). Antifungal and anti-inflammatory potential of Bupleurum rigidum subsp. Paniculatum (Brot.) H. Wolff essential oil. Antibiotics (Basel), 10(5), 592.

Chemical constituents and antimicrobial ability of essential oil from the fruits of Lonicera maackii (Rupr.) Maxim.

Abstract

References

Most read articles by the same author(s)